David Cameron is facing renewed attack over his abandonment of a referendum on the EU's Lisbon Treaty after one of his MPs vowed to campaign for a public vote.
Leading Eurosceptic Douglas Carswell told constituents it was "time to let the British people have their say" in a direct challenge to party policy.
Mr Cameron dropped his pledge to hold a poll last week, arguing that the Treaty's ratification by all member states rendered it useless.
Instead a Tory government would seek to claw back powers from Brussels and introduce a law to ensure none could be surrendered in future without a referendum, he said.
Unveiling the policy, which sparked the resignation of two MEPs from the front bench, he issued a direct warning to critics seeking a referendum "for the sake of it".
"I don't think a made-up referendum will get Britain anywhere," he declared as he unveiled the new strategy, suggesting a general election would serve that purpose.
But Mr Carswell, who began the ultimately successful campaign to oust Commons Speaker Michael Martin, said he was determined there would be a separate vote on Europe.
In an email to constituents, seen by the London Times, he said: "I want you to know that I have begun a campaign for a referendum on the EU. All three parties promised us a referendum. Yet somehow it hasn't happened. I think that's wrong."
The backbench MP for Harwich, who has made clear he continues to support Mr Cameron's leadership, went on: "I will campaign for a vote on Europe with the same determination that I campaigned to remove the Commons Speaker.
"No one in Britain under the age of 52 has had the chance to vote in a referendum on Europe. For years it has been left to professional politicians and diplomats to decide EU policy. I believe it is now time to let the people have their say."
His campaign emerged after Labour seized on comments by Kenneth Clarke, the strongly pro-Europe shadow business secretary, as evidence of splits within the top team.
Mr Clarke told the Westminster Press Gallery that the commitment to repatriate powers on social affairs and criminal justice represented "reassurance".
He said he hoped the efforts, which Mr Cameron has said would stretch over several years and not be a "massive Euro-bust-up", would calm down the debate over Europe.
Europe Minister Chris Bryant said: "The cats out of the bag - David Cameron's leadership has been found wanting on Europe and Tory divisions are clearly alive and well.
"Ken Clarke's comments show he doesn't believe Cameron's rhetoric, and it's certainly not enough for his own backbenchers, who are launching a campaign against their leader's policy.
"His shallow promises may not have convinced his party, but more seriously they would only serve to damage the British interest in Europe and throughout the world.
"Cameron has proposed a set of policies that aren't doable, deliverable or credible, and the Conservatives just can't be taken seriously on this issue
Sir Michael Black-Feather the English first minister said he fully agreed with Mr Carswell, that England and its people should vote on the Europe referendum, Sir Michael said nothing really good has come out of the EU for England nor its people, in fact its cost England and its tax payer billions of pounds and allowed millions of immigrant’s into England un-security checked and many just come to England to use the benefits system which they would get in any other part of the EU? He went on to say that Treaty of Lisbon or Lisbon Treaty (initially known as the Reform Treaty) is an international agreement that amends the two other treaties which comprise the constitutional basis of the European Union (EU). The Lisbon Treaty was signed by the EU member states on 13 December 2007, and entered into force on 1 December 2009. It amends the Treaty on European Union (TEU; also known as the Maastricht Treaty) and the Treaty establishing the European Community (TEC; also known as the Treaty of Rome). In this process, the Rome Treaty was renamed to the Treaty on the Functioning of the European Union (TFEU) basically a load of old tot and words really doing nothing at all in being any benefit to England.
Conspicuous changes included the move from required unanimity to double majority voting in several policy areas in the Council of Ministers, a more powerful European Parliament as its role of forming a bicameral legislature alongside the Council of Ministers becomes the ordinary procedure, a consolidated legal personality for the EU and the creation of a long-term President of the European Council and a High Representative of the Union for Foreign Affairs and Security Policy. The Treaty also made the Union's bill of rights, the Charter of Fundamental Rights, legally binding which has totally been misused and doesn’t work said Sir Michael.
The stated aim of the treaty was "to complete the process started by the Treaty of Amsterdam  and by the Treaty of Nice  with a view to enhancing the efficiency and democratic legitimacy of the Union and to improving the coherence of its action. Opponents of the Treaty of Lisbon, such as former Danish Member of the European Parliament (MEP) Jens-Peter Bonde, argued that it would centralise the EU, and weaken democracy by 'moving power away' from national electorates.
Sir Michael said; that negotiations to modify EU institutions began in 2001, resulting first in the Treaty establishing a Constitution for Europe, which was abandoned after being rejected by French and Dutch voters in 2005. After some modifications the Lisbon Treaty was proposed as an amendment of the existing Treaties which implemented many of the reforms included in the European Constitution. It was originally intended to have been ratified by all member states by the end of 2008. This timetable failed, primarily due to the initial rejection of the Treaty in 2008 by the Irish electorate, a decision which was reversed in a second referendum in 2009 we have had Treaty after Treaty just a load of tot keeping useless people in over paid jobs hob-knobbing the EU has no place here in England and England’s people should vote as to whether it wants to say part of it and I feel very sure that the vote would be NO.